In her presentation on "Efforts and Resources for Addressing Antimicrobial Resistance," Dr. Jean Patel,, Science Lead, ARX Unit,, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, describes potential use of digital dispensing inkjet technology for establishing on-demand MIC testing at ALRN labs. Dr. Patel's slide presentation is available from the FDA website.
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Postdoctoral Fellow Thea Brennan-Krohn becomes diplomate of American Board of Medical Microbiology8/19/2017 Congratulations to Thea Brennan-Krohn for passing her ABMM exam and becoming a diplomate of the American Board of Medical Microbiology
Smith KP, Richmond DL, Brennan-Krohn T, Elliott HL, Kirby JE. Development of MAST: a Microscopy-Based Antimicrobial Susceptibility Testing Platform. SLAS Technology, In press.
Use of inkjet printing, advanced imaging, and machine learning to achieve reference standard, microdilution antimicrobial susceptibility testing readout in two hours with off-the-shelf supplies. For any antimicrobial at will. Update: Now Published On-Line in SLAS Technology Website!
.On acceptance of her manuscript by the Journal of Antimicrobial Chemotherapy:
Brennan-Krohn T, Truelson KA, Smith KP, Kirby JE. Screening for synergistic activity of antimicrobial combinations against carbapenem-resistant Enterobacteriaceae using inkjet printer-based technology. J Antimicrobial Chemotherapy. 2017 July. Link to abstract. Link to Journal Full Text. Please read this informative blog post by clinical microbiology and postdoctoral fellow, Thea Brennan-Krohn: "AST FOR NEW ANTIBIOTICS: THE CLINICAL LABORATORIAN'S DILEMMA."
Our antimicrobial susceptibility testing research is highlighted on Harvard Catalyst Spotlight3/6/2017 See discussion of our work on Microscopy-Based Antimicrobial Susceptibility Testing as part of the Harvard Catalyst Reactor Pilot Program.
In the Journal of Clinical Microbiology, we describe an unusual Candida pseudo-outbreak that based on patterning on culture plates mimicked true infection. Specifically, colonies were not randomly distributed on plates, but demonstrated in a graded pattern typical of true infections with decreasing number of colonies on subsequent quandrants of streak plates. The culprit was eventually identified, as a contaminated Anoxomat chamber that was used to hold anaerobic plates prior to use in cultures. The Anoxomat rapidly purges and instills a hydrogen/nitrogen gas mixture into a closed jar in order to rapidly establish anaerobiosis. We believe that the Anoxomat overcame the natural swan neck barrier of Petri dishes allowing Candida guilliermondii containing aerosols to randomly contaminate anaerobic plates. C. guilliermondii does not grow anaerobically obscuring this contamination. However, randomly contaminated anaerobic plates were subsequently the source of contamination for the primary quandrants of aerobic media during specimen planting leading to simulation of true infection by microbiological criteria. Pseudo-outbreak strains were proven clonal by a combination of pulsed-field gel electropheresis and optical restriction mapping. The study represented a combined effort of the clinical microbiology laboratory and the infection control/hospital epidemiology divisions at BIDMC; Dan Diekema at the University of Iowa (PFGE analysis and expertise); and OpGen (optical mapping).
I was selected as a SLAS 2017 Innovation Award Finalist and will be speaking at the SLAS Annual Meeting with a presentation titled: "Inkjet Printing Technology for Facilitated At Will Antimicrobial Susceptibility Testing (FAST) in Under 5 Hours: Addressing the Needs of a So-Called 'Post-Antibiotic Era'.
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