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I was selected as a SLAS 2017 Innovation Award Finalist and will be speaking at the SLAS Annual Meeting with a presentation titled: "Inkjet Printing Technology for Facilitated At Will Antimicrobial Susceptibility Testing (FAST) in Under 5 Hours: Addressing the Needs of a So-Called 'Post-Antibiotic Era'.
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Co-First Authors, KP Smith and Thea Brennan-Krohn, from the Kirby Research Laboratory and Susan Weir from the BIDMC Clinical Microbiology Laboratory collaboratively investigated the ability of commonly used clinical methods to support new "susceptible dose-dependent." MIC breakpoints newly introduced by the Clinical Laboratoryand Standards Institute for the antibiotic cefepime. The idea behind these SDD breakpoints was to offer clinicians the chance to treat otherwise poorly susceptible pathogens by increasing the cefepime dose in a manner tied to the isolate's MIC. To perform the study, we enriched for strains that should have borderline cefepime susceptibility based on a ceftriaxone resistant phenotype. Surprisingly, three commercial methods (Vitek 2, disk diffusion, and a manual microscan panel) performed poorly with only 40-60% categorical agreement with the broth microdilution reference standard. In contrast, the Digital Dispensing Method, (DDM) previously described by our laboratory was stastically equivalent to the reference method, and therefore was the only method capable of supporting susceptible dose dependent therapeutic rescue. The manuscript, "Improved Accuracy of Cefepime Susceptibility Testing for ESBL-producing Enterobacteriaceae using an On-Demand Digital Dispensing Method" can be found on the Journal of Clinical Microbiology website.
I am looking forward to giving a podium and poster presentation at the ASM Conference on Antibacterial Development. I will be discussing our efforts to accelerated antimicrobial susceptibility testing diagnostics. More rapid and flexible AST diagnostics are critical for clinical introduction and for directing appropriate use of new antimicrobials under development.
In an article posted online today in Future Microbiology titled "How inkjet printing technology can defeat multidrug-resistant pathogens", postdoctoral fellow, KP Smith, and I discuss the potential uses of inkjet printing digital dispensing technology for addressing the antimicrobial susceptibility testing gap.LRIG stands for laboratory robotics interest group. My talk was titled: "How inkjet printing technology can defeat multidrug-resistant superbugs." In addition to a number of platform presentations, the meeting featured a large exhibit hall with various types of research laboratory automation on display.
More details to follow. More about the meeting can be found at www.slas2017.org. . More information about SLAS can be found at www.slas.org.
BIDMC issued a press release on our manuscript on at-will antimicrobial susceptibility testing performed using a digital dispensing method. "Simple Method Quickly Tests Hard-to-Treat Bacteria’s Susceptibility to Different Antibiotics".
The original article is now available online at: http://www.ncbi.nlm.nih.gov/pubmed/27335151 Postdoctoral Fellow, KP Smith, describes the use of inkjet printing technology to perform antimicrobial susceptibility testing of any antimicrobial at will. Importantly, the method is extremely simple, requiring one human pipetting step per antimicrobial, and is just as accurate and more precise than the reference gold standard broth microdilution method. The findings were reported in the Journal of Clinical Microbiology.
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