The Kirby Laboratory
  • Home
  • Antimicrobials
    • Legionella pneumophila
    • T4SS-dependent Gram negative pathogens
    • CRE Pathogens
    • Mechanisms of carbapenem resistance >
      • Carbapenemases | KPC | NDM-1
      • Porins
      • Efflux pumps
  • Diagnostics
  • SARS-CoV-2
  • Pathogenesis
  • About the PI
  • Personnel
  • Publications
  • Blog
  • Environment
  • Positions
  • Resources
  • Donations
  • Comments
  • Contact Info
  • Internal Lab Resources

"Improved Accuracy of Cefepime Susceptibility Testing for ESBL-producing Enterobacteriaceae using an On-Demand Digital Dispensing Method" published online yesterday in the Journal of Clinical Microbiology

12/2/2016

2 Comments

 
Co-First Authors, KP Smith and Thea Brennan-Krohn, from the Kirby Research Laboratory and Susan Weir from the BIDMC Clinical Microbiology Laboratory collaboratively investigated the ability of commonly used clinical methods to support new "susceptible dose-dependent." MIC breakpoints newly introduced by the Clinical Laboratoryand Standards Institute for the antibiotic cefepime. The idea behind these SDD breakpoints was to offer clinicians the chance to treat otherwise poorly susceptible pathogens by increasing the cefepime dose in a manner tied to the isolate's MIC.  To perform the study, we enriched for strains that should have borderline cefepime susceptibility based on a ceftriaxone resistant phenotype.  Surprisingly, three commercial methods (Vitek 2, disk diffusion, and a manual microscan panel) performed poorly with only 40-60% categorical agreement with the broth microdilution reference standard.  In contrast, the Digital Dispensing Method, (DDM) previously described by our laboratory was stastically equivalent to the reference method, and therefore was the only method capable of supporting susceptible dose dependent therapeutic rescue. The manuscript, "Improved Accuracy of Cefepime Susceptibility Testing for ESBL-producing Enterobacteriaceae using an On-Demand Digital Dispensing Method" can be found on the Journal of Clinical Microbiology website.
2 Comments

    Kirby Lab Blog

    ​

    Follow @KirbyLabMicrobe

    RSS Feed

    Categories

    All
    Acinetobacter
    Antibiotics
    Antimicrobial Resistance
    Antimicrobial Susceptibility Testing
    Apramycin
    Artificial Intelligence
    ASM Blog
    Awards
    Brucella
    Candida Auris
    Carbapenem-Resistant Acinetobacter Baumannii (CRAB)
    Carbapenem Resistant Enterobacteriaceae
    Carbapenem-Resistant Enterobacteriaceae
    Clinical Microbiology
    Colistin
    Collaborations
    Images
    Inkjet Printing Diagnostics
    Inoculum Effect
    Klebsiella
    Lab Activities
    Lab Member News
    Legionella
    Manuscripts
    Medicinal Chemistry
    Meetings
    Microbiology And Society
    Microbiology General Interest
    MRSA
    News Release
    Postdoctoral Research
    Post Graduate Research
    Post-Graduate Research
    Staphylococcus Aureus
    Synergy
    Type IV Secretion System
    Undergraduate Research
    VISA

    Archives

    March 2023
    February 2023
    December 2022
    August 2022
    April 2022
    January 2022
    December 2021
    September 2021
    July 2021
    April 2021
    March 2021
    February 2021
    January 2021
    November 2020
    September 2020
    July 2020
    May 2020
    April 2020
    March 2020
    February 2020
    November 2019
    October 2019
    September 2019
    July 2019
    June 2019
    April 2019
    March 2019
    February 2019
    January 2019
    December 2018
    November 2018
    October 2018
    September 2018
    August 2018
    July 2018
    June 2018
    May 2018
    April 2018
    March 2018
    February 2018
    January 2018
    December 2017
    November 2017
    October 2017
    September 2017
    August 2017
    July 2017
    March 2017
    January 2017
    December 2016
    November 2016
    October 2016
    September 2016
    July 2016
    June 2016
    May 2016
    April 2016
    March 2016
    December 2014

    Antimicrobials
    Diagnostics
    Pathogenesis
    About the PI
    Personnnel
    Donate
  • Home
  • Antimicrobials
    • Legionella pneumophila
    • T4SS-dependent Gram negative pathogens
    • CRE Pathogens
    • Mechanisms of carbapenem resistance >
      • Carbapenemases | KPC | NDM-1
      • Porins
      • Efflux pumps
  • Diagnostics
  • SARS-CoV-2
  • Pathogenesis
  • About the PI
  • Personnel
  • Publications
  • Blog
  • Environment
  • Positions
  • Resources
  • Donations
  • Comments
  • Contact Info
  • Internal Lab Resources