New combinations and repurposed antibiotics active against the pandrug-resistant Nevada CRE, NDM-1 strain early release today in AAC
Our latest manuscript: "Synergistic combinations and repurposed antibiotics active against the pandrug-resistant Klebsiella pneumoniae Nevada strain." Antimicrobial Agents and Chemotherapy early release identifies:
Highly synergistic activity of several antimicrobial combinations.
Low apramycin and spectinomycin MIC values.
CDC previously reported that none of 26 antimicrobials they tested were active against the Nevada strain. That made the strain until now pandrug-resistant.
Links to several news articles about this pandrug-resistant strain::
The Atlantic. NPR, NBC, PBS. Forbes
"3 Drug Combinations Appear Effective Against C auris" by Jared Kaltwasser discussed work presented by Thea Brennan-Krohn at ASM Microbe 2019.
There is a very fancy drawing of a single cell life form on the slide undoubtedly being compromised by a combination of antimicrobial therapeutics applied in Thea, Liam, and Sarah's experiments!
Postdoctoral Fellow Yoon-Suk Kang's Brucella chemical genetics screen early release today in Infection and Immunity
Congratulations to Yoon-Suk on his latest Brucella neotomae manuscript: "A chemical genetics screen reveals influence of p38 map kinase and autophagy on phagosome development and intracellular replication of Brucella neotomae in macrophages"
Congratulions to postdoctoral fellow, KP Smith, for being selected for the Paul E Strandjord Young Investigator Award
KP's abstract was selected for the Paul E Strandjord Young Investigator Award from the Academy of Clinical Laboratory Physicians and Scientists (ACLPS). He will be given an oral presentation at the ACLPS annual meeting this coming June. ACLPS is the organization that represents and promotes the discipline of academic clinical pathologists. Congratulations KP!
ASM has reorganized their website. Their blogs are now impossible to find, They are now organized under author pages. Blogs from current and former research and/or clinical fellows:
KP Smith's blogs can be found at: www.asm.org/Biographies/Kenneth-(K-P-)-Smith
Rose Lee's blogs can be found at: www.asm.org/Biographies/Rose-Lee
Thea Brennan-Krohn's blogs can be found at: www.asm.org/Biographies/Thea-Brennan-Krohn
Jennifer Tsang's blogs can be found at: www.asm.org/Biographies/Jennifer-Tsang
Congratulations to Kate Zulauf for Peggy Carter Travel Award to attend the ASM Microbe Meeting in San Francisco, CA!
The ASM Peggy Cotter Travel Award Program provides funds for early career branch members, in this case, the ASM Northeast Branch, to attend the ASM Microbe Meeting, to be held this year in San Francisco, CA. Kate will be presented her work on a small molecule screen to evict CRE resistance plasmids.
JoVE manuscript accepted on use of Inkjet Printing to perform antimicrobial susceptibility testing for single and drug combinations and follow up time-kill study methodology
Postdoctoral fellow, Thea Brennan-Krohn, recently had a manuscript accepted in the Journal of Visualized Experimentation, aka JoVE. The title of the manuscript and link to the abstract are "Antimicrobial Synergy Testing by the Inkjet Printer-Assisted Automated Checkerboard Array and the Manual Time-Kill Method." We have been fielding a lot of questions over the past two years about implementation of inkjet printing antimicrobial susceptibility testing technology and thought it would be useful to share a video of the technique as well as classic time-kill analysis to analyze antimicrobial synergy. We are excited to learn that the CDC has decided to implement the technology in the near future in their Antimicrobial Resistance Laboratory Network (ARLN), initially to test, the combination of ceftazidime-avibactam and aztreonam for activity against multidrug-resistant Gram-negatives.
KP Smith Speaking tomorrow at Harvard Antimicrobial Resistance Laboratory Network as well as Broad Institute collaborator, Alejandro Pironti
Congratulations to postdoctoral fellow, Yoon-Suk Kang, on acceptance of his manuscript in Infection and Immunity. The manuscript is titled: "Brucella neotomae recapitulates attributes of zoonotic human disease in a murine infection model." One of my favorite figures are images of tdTomato-labeled Brucella detected by confocal microscopy replicating in the middle of a liver granulomas visualized by counterstaining with H&E. In contrast to wild type organisms, virB4 mutant bacteria are found as single cells in sinusoids presumably within Kuffpfer cells. Wild type bacteria are found at high levels in mouse thymus at all time points examined; virB4 mutant bacteria are undetectable in thymus. B. neotomae induces a Th-1 immune response. More information in the manuscript.
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