Congratulations to Postdoctoral Fellow Yoon-Suk Kang on publication of his manuscript "Promotion and Rescue of Intracellular Brucella neotomae Replication During Co-Infection With Legionella pneumophila"
Published online today in Infection and Immunity: "Promotion and Rescue of Intracellular Brucella neotomae Replication During Co-Infection With Legionella pneumophila." Yoon-Suk created a versatile bioreporter toolkit to enable analysis of the fate of individual pathogens in polymicrobial infections. He then validated a type IV secretion system-dependent Brucella model using the wood rat pathogen, Brucella neotomae. In contrast to wild type organisms, Brucella T4SS mutants were completely defective in their ability to growth inside of macrophages. Fascinatingly co-infection with Legionella pneumophila, another T4SS-dependent pathogen, was able to rescue intracellular growth of the T4SS-mutant Brucella and also stimulated growth of wild type Brucella organisms! This was a one way rescue: Legionella could rescue Brucella, but wild type Brucellla could not rescue T4SS-defective Legionella.
"High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity" published today in JoVE
Lucius, Yoon-Suk and I had fun filming this article in our tissue culture area and at the ICCB-Longwood High Throughput Screening Facility. Here is the link: "High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity." Legionella pneumophila was received an Academy Award for best supporting role.
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