New combinations and repurposed antibiotics active against the pandrug-resistant Nevada CRE, NDM-1 strain early release today in AAC
Our latest manuscript: "Synergistic combinations and repurposed antibiotics active against the pandrug-resistant Klebsiella pneumoniae Nevada strain." Antimicrobial Agents and Chemotherapy early release identifies:
Highly synergistic activity of several antimicrobial combinations.
Low apramycin and spectinomycin MIC values.
CDC previously reported that none of 26 antimicrobials they tested were active against the Nevada strain. That made the strain until now pandrug-resistant.
Links to several news articles about this pandrug-resistant strain::
The Atlantic. NPR, NBC, PBS. Forbes
"3 Drug Combinations Appear Effective Against C auris" by Jared Kaltwasser discussed work presented by Thea Brennan-Krohn at ASM Microbe 2019.
There is a very fancy drawing of a single cell life form on the slide undoubtedly being compromised by a combination of antimicrobial therapeutics applied in Thea, Liam, and Sarah's experiments!
A primer on use of inkjet printing and time-kill studies for combination antimicrobial susceptibility testing
Adopted by the CDC .in their Antimicrobial Resistance Laboratory Network based on our prior publications in Journal of Clinical Microbiology, Journal of Antimicrobial Chemotherapy, and Antimicrobial Agents and Chemotherapy for testing of aztreonam/ceftazidime-avibactam combination activity using inkjet printing. We provide a step by step video demonstration of assay setup in the Journal of Visualized Experimentation. Link to article landing page (PDF protocol access and video), "Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method" by Thea Brennan-Krohn and James E. Kirby.
JoVE manuscript accepted on use of Inkjet Printing to perform antimicrobial susceptibility testing for single and drug combinations and follow up time-kill study methodology
Postdoctoral fellow, Thea Brennan-Krohn, recently had a manuscript accepted in the Journal of Visualized Experimentation, aka JoVE. The title of the manuscript and link to the abstract are "Antimicrobial Synergy Testing by the Inkjet Printer-Assisted Automated Checkerboard Array and the Manual Time-Kill Method." We have been fielding a lot of questions over the past two years about implementation of inkjet printing antimicrobial susceptibility testing technology and thought it would be useful to share a video of the technique as well as classic time-kill analysis to analyze antimicrobial synergy. We are excited to learn that the CDC has decided to implement the technology in the near future in their Antimicrobial Resistance Laboratory Network (ARLN), initially to test, the combination of ceftazidime-avibactam and aztreonam for activity against multidrug-resistant Gram-negatives.
KP Smith Speaking tomorrow at Harvard Antimicrobial Resistance Laboratory Network as well as Broad Institute collaborator, Alejandro Pironti
"A Little Change Can Make a Lot of MIC Difference: the Inoculum Effect and Antibiotic Susceptibility Testing"
Thanks to American Society of Microbiology science writer, Julie Wolf, for highlighting our inoculum effect manuscript in the ASM mBiosphere Blog, "A Little Change Can Make a Lot of MIC Difference: the Inoculum Effect and Antibiotic Susceptibility Testing"
Synergistic Activity of Colistin-Containing Combinations against Colistin-Resistant Enterobacteriaceae.
Published online today in Antimicrobial Agents and Chemotherapy! Thea Brennan-Krohn, Alejandro Pironti, and James E. Kirby. Synergistic Activity of Colistin-Containing Combinations against Colistin-Resistant Enterobacteriaceae. Antimicrob. Agents Chemother. Accepted manuscript posted online 30 July 2018 , doi:10.1128/AAC.00873-18.
Now live on the Antimicrobial Agents and Chemotherapy Webpage: The Inoculum Effect in the Era of Multidrug Resistance: Minor Differences in Inoculum Have Dramatic Effect on MIC Determination.
Kirby Lab Blog