Gentian violet is diluted in hollow fiber cassette with a half life of approximately 4 hours based on dilution of initial "dose" in central compartment with fresh media at flow rate selected on peristaltic pump. Next two antibiotics with different half lives. Will the bacteria outside the hollow fibers survive and for how long?
We have been taking advantage of Addgene for years. About to deposit about twenty plasmids form Yoon-Suk Kang's bioreporter tool kit. Assembling plasmid maps in SnapGene. Thank you, Addgene!
Excel and Powerpoint have been banned for figure preparation. Converted my lab over to GraphPad Prism. A very well designed graphing and statistics program. Love several features including the ability to apply standard dimension formats for charts, and wand tool to covert charts to exact format used previously. Application of statistics to data is facile, also a wand tool to apply the same statistics to multiple tables. Grateful to Assay and Drug Development Technology for their requirement (recommendation?) to use this or similar programs in figure preparation. Powerpoint never worked well for figure preparation and conversion to vector-graphics format, for use with illustrator was a nightmare. Now we just start out using a freeware vector graphics program or Adobe Illustrator directly. Greatly speeds up manuscript preparation.
P.S. I have no financial relationship with GraphPad Prism, but it is a good product so therefore my endorsement!
Our Gram stain artificial intelligence platform was selected as a competitor in the 2018 Stat Madness Competition.
Work from our laboratory was selected as a competitor in the 2018 Stat Madness Competition. This bracket style national competition will allow voters to choose among important medical innovations developed at selected medical centers and universities across the United States. Our artificial intelligence-based-platform named Technologist Assist allows automatic imaging and interpretation of Gram stain results and ultimately will provide assisted laboratory diagnostic capabilities at near or remote sites.
Please vote for our work at the Stat Madness website: www.statnews.com/feature/stat-madness/bracket/
We officially moved the lab today. Not very far. Just to other side of the floor. Doubled our bench space! We are all very excited!
Research Associate Position | MAST | Beth Israel Deaconess Medical Center | Boston | Kirby Laboratory | Harvard Medical School
Seeking to hire a research associate to work on the MAST project (microscopy-based antimicrobial susceptibility testing. Please contact firstname.lastname@example.org and send a c.v., short statement of interest, and contact information for three references. The official BIDMC official posting is in the described as a research assistant in the pathology department.
Image from lab meeting! Spinning disk confocal microscope image of B. neotomae and J744A.1 macrophages
Spinning disc confocal microscopy image acquired by Lucius Chiaraviglio and presented at Lab Meeting this past Friday. A single multiply infected macrophage is shown. Red objects are individual Brucella neotomae bacteria. Green immunofluorescent staining lables LAMP-1, a marker of late endosomes and lysosomes. Brucella traffics to LAMP-1 compartments early during infection.
Kirby Lab Blog