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"3 Drug Combinations Appear Effective Against C auris" by Jared Kaltwasser discussed work presented by Thea Brennan-Krohn at ASM Microbe 2019.
 There is a very fancy drawing of a single cell life form on the slide undoubtedly being compromised by a combination of antimicrobial therapeutics applied in Thea, Liam, and Sarah's experiments!
Congratulations to Matt on admission to medical school and Liam for admission to graduate school!!!
The ASM Peggy Cotter Travel Award Program provides funds for early career branch members, in this case, the ASM Northeast Branch, to attend the ASM Microbe Meeting, to be held this year in San Francisco, CA. Kate will be presented her work on a small molecule screen to evict CRE resistance plasmids.
 Gentian violet is diluted in hollow fiber cassette with a half life of approximately 4 hours based on dilution of initial "dose" in central compartment with fresh media at flow rate selected on peristaltic pump. Next two antibiotics with different half lives. Will the bacteria outside the hollow fibers survive and for how long?
We have been taking advantage of Addgene for years. About to deposit about twenty plasmids form Yoon-Suk Kang's bioreporter tool kit. Assembling plasmid maps in SnapGene. Thank you, Addgene!
Excel and Powerpoint have been banned for figure preparation. Converted my lab over to GraphPad Prism. A very well designed graphing and statistics program. Love several features including the ability to apply standard dimension formats for charts, and wand tool to covert charts to exact format used previously. Application of statistics to data is facile, also a wand tool to apply the same statistics to multiple tables. Grateful to Assay and Drug Development Technology for their requirement (recommendation?) to use this or similar programs in figure preparation. Powerpoint never worked well for figure preparation and conversion to vector-graphics format, for use with illustrator was a nightmare. Now we just start out using a freeware vector graphics program or Adobe Illustrator directly. Greatly speeds up manuscript preparation.
P.S. I have no financial relationship with GraphPad Prism, but it is a good product so therefore my endorsement!  Staining with LAMP-1, a lysosomal marker  Green fluorescent latex beads  Merged image. Note latex beads are surrounded by LAMP-1 and are therefore in lysosomal compartments. Far-Red staining of LAMP-1 reduces amount of background autofluorescence  Lab art with HP D300 digital dispensing system. Crystal violet blue, phenol red, background droplets. |
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