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Kirby Lab Outing to Tiger Mama, Boston, MA

7/21/2019

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Latex beads enter lysosomal pathway in J774A.1 macrophages - LAMP-1 staining

3/19/2018

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Staining with LAMP-1, a lysosomal marker
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Green fluorescent latex beads
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Merged image. Note latex beads are surrounded by LAMP-1 and are therefore in lysosomal compartments. Far-Red staining of LAMP-1 reduces amount of background autofluorescence
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Fun with Inkjet Printer

3/15/2018

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Lab art with HP D300 digital dispensing system. Crystal violet blue, phenol red, background droplets.
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Newest Member of the Laboratory (GEOFORCE GTX TITAN Xp) - thank you NVIDIA!

2/26/2018

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GEOFORCE GTX TITAN Xp in its new home
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Image from lab meeting!  Spinning disk confocal microscope image of B. neotomae and J744A.1 macrophages

12/16/2017

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Spinning disc confocal microscopy image acquired by Lucius Chiaraviglio and presented at Lab Meeting this past Friday.  A single multiply infected macrophage is shown.  Red objects are individual Brucella neotomae bacteria. Green immunofluorescent staining lables LAMP-1, a marker of late endosomes and lysosomes.  Brucella traffics to LAMP-1 compartments early during infection.  

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KP Smith Poster Talk at ASM Microbe

9/30/2017

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KP Smith Presents MAST Inkjet Technology at 2017 ASM Microbe Meeting
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MAST:  Microscopy-Based Antimicrobial Susceptibility Testing now at local grocery store

8/20/2017

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"High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity" published today in JoVE

11/16/2016

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Lucius, Yoon-Suk and I had fun filming this article in our tissue culture area and at the  ICCB-Longwood High Throughput Screening Facility.  Here is the link:  "High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity."  Legionella pneumophila was received an Academy Award for best supporting role.
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  • Home
  • Antimicrobials
    • Legionella pneumophila
    • T4SS-dependent Gram negative pathogens
    • CRE Pathogens
    • Mechanisms of carbapenem resistance >
      • Carbapenemases | KPC | NDM-1
      • Porins
      • Efflux pumps
  • Diagnostics
  • SARS-CoV-2
  • Pathogenesis
  • About the PI
  • Personnel
  • Publications
  • Blog
  • Environment
  • Positions
  • Resources
  • Major Equipment
  • Donations
  • Comments
  • Contact Info
  • Internal Lab Resources