Image from lab meeting! Spinning disk confocal microscope image of B. neotomae and J744A.1 macrophages
Spinning disc confocal microscopy image acquired by Lucius Chiaraviglio and presented at Lab Meeting this past Friday. A single multiply infected macrophage is shown. Red objects are individual Brucella neotomae bacteria. Green immunofluorescent staining lables LAMP-1, a marker of late endosomes and lysosomes. Brucella traffics to LAMP-1 compartments early during infection.
"High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity" published today in JoVE
Lucius, Yoon-Suk and I had fun filming this article in our tissue culture area and at the ICCB-Longwood High Throughput Screening Facility. Here is the link: "High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity." Legionella pneumophila was received an Academy Award for best supporting role.
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