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Congratulations to Minte and colleagues!

1/1/2025

6 Comments

 
Our New Year's Eve manuscript acceptance, full text posting on ACS Infectious Diseases.  Congratulations to members of the Manetsch and Kirby Laboratories on on line publication of: "Development of CGS-15943 Adjunctives for the Disruption of Plasmid Maintenance in Multidrug Resistant E. coli." 

The manuscript describes structure activity relationship studies on a small molecule scaffold  CGS-15943.  The parent compound causes complete eviction of an IncFIA plasmid with highly selectivity in low, single-digit micromolar concentrations.   Kate Zulauf at al. previously described identification and characterization of several potent plasmid eviction compounds in our PNAS publication.  In our ACS Infectious Diseases manuscript, one portion of MInte's PhD thesis, CGS-15943 analogs were identified which were more potent than the parent compound, and with increased penetrance, An exciting step forward in our plasmid eviction work.
6 Comments

Undergraduate, Heidi Alhannat, takes over tissue culture incubator for natural product production

7/13/2024

5 Comments

 
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Forget CO2, Forget Temperature.
Selman Waksman  step over!  A big thumbs up! 

5 Comments

SARS-CoV-2 Clinical Microbiology Review - just pubished

3/19/2024

5 Comments

 
5 Comments

Welcome Ariane Paz y Mino who will be joining the lab part time later in January

1/9/2024

1 Comment

 
Ariane was immersed in antimicrobial resistance research on gram negatives in Ecuador prior to starting an internal medicine residency in 2022 at Salem Hospital and MGB.  In her spare time during her medical training, she will be continuing her investigation of gram-negative resistance mechanism in the Kirby lab.
1 Comment

Manetsch Laboratory Presents New Streptothricin Data at BAARN 2023

12/17/2023

1 Comment

 
BAARN would not be complete without antimicrobial medicinal chemistry.  Roman Manetsch and lab were there in force to highlight collaborative efforts on the streptothricin scaffold.
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Left to Right: Roman Manetsch, Brandon Miller, Yuliya Marusyk
1 Comment

Kirby and Brennan-Krohn Labs Present at BAARN

12/17/2023

0 Comments

 
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Kirby and Brennan-Krohn Labs at BAARN 2023 Meeting, Poster Session Memorial Hall, Harvard College. Left to Right: Yanqin Huang, Michelle Nägeli, Anusha Shukla, James Kirby, Alhanouf Aljahdali, Thea Brennan-Krohn, Matt Hernandez
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Our PloS Bio article is out!  Streptothricin-F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome

5/17/2023

0 Comments

 
The Plos Bio Article
Some Press Releases:
Researchers Show an Antibiotic Discovered 80 Years Ago Is Effective Against Multi-Drug Resistant Bacteria

Neglected 80-year-old antibiotic is effective against multi-drug resistant bacteria

Vergeten antibioticum kan uitkomst zijn in strijd tegen multiresistente bacteriën
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Streptothricin-F (yellow spheres) bound to 16S rRNA (green) of the bacterial ribosome impinges on the decoding site where the anti-codon stem loop of the A-site tRNA (purple) binds to the codon of the mRNA (blue). This bumping of streptothricin-F into the decoding site is associated with translational infidelity (insertion of amino acids into the growing peptide chain other than those encoded for by the mRNA), ultimately leading to poisoning and death of the bacterial cell. The image was created using Pymol by alignment of PDB 7UVX containing streptothricin-F (this manuscript) with the E. coli 70S ribosome from PDB 7K00 containing mRNA and the A-site tRNA shown (DOI: 10.7554/eLife.60482).
0 Comments

Postdoctoral Position Available - Kirby Laboratory

4/11/2023

0 Comments

 
General areas include antimicrobial resistance, antimicrobial discovery, novel methods of treating antimicrobial resistant pathogens. 
0 Comments

Postdoctoral Fellowship Position Available Immediately in the Laboratory of Ed Yu for Collaborative Studies to Investigate Interaction of Small Molecules with the Ribosome and RNA

4/2/2023

0 Comments

 


A postdoctoral position is available immediately (4/2/2023) in the Laboratory of Ed Yu to study biology of the ribosome; mechanism of action of translational inhibitors; and interaction of small molecules with RNA in the Department of Pharmacology at Case Western Reserve. Projects are being performed collaboratively with the Kirby, Manetsch, and O'Doherty Laboratories. See recent manuscripts (
PMC8805024, PMC8263017, PMC6974574)

The incoming research fellow will use x-ray crystallography/cryo-EM and other biophysical/biochemical techniques to elucidate the molecular mechanisms in these systems. Please find additional information about the Yu group and the outstanding training environment in the Case Western Department of Pharmacology website (https://case.edu/medicine/pharmacology/).

This position requires expertise in structural biology. The ideal candidate will have shown the ability to guide a project to completion, from protein expression to structural analysis. Knowledge of common structural programs (Phenix, Coot, Pymol) a must. Experience with cryo-electron microscopy sample preparation/operation/data analysis is highly desirable. Experience with single-molecule FRET and other biochemical and biophysical characterization methods would also be considered a strength.

Qualified applicants must have a PhD or MD/PhD degree; strong written, oral communication, and critical thinking skills are required. As a Postdoctoral Scholar, he/she will present data at departmental seminars, prepare manuscripts, and contribute preliminary data for grant proposals. Preference will be given to applicants with a recognized record of accomplishment as evidenced by scholarly publications in the field of bacteriology or structural biology. Send inquiries containing a cover letter, CV with list of publications and contact information to Dr. Edward Yu ([email protected]).

See also Ed' posting

0 Comments

RND Pump-Mediated Efflux of Amotosalen, a Compound Used in Pathogen Inactivation Technology to Enhance Safety of Blood Transfusion Products, May Compromise Its Gram-Negative Anti-Bacterial Activity:

3/3/2023

0 Comments

 
Just published in mSphere!  Images from our manuscript show below.  Modeling of amotosalen efflux from the A. baumannii AdeB efflux pump.  Ref:
DOI: https://doi.org/10.1128/msphere.00673-22
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  • Home
  • Antimicrobials
    • Legionella pneumophila
    • T4SS-dependent Gram negative pathogens
    • CRE Pathogens
    • Mechanisms of carbapenem resistance >
      • Carbapenemases | KPC | NDM-1
      • Porins
      • Efflux pumps
  • Diagnostics
  • SARS-CoV-2
  • Pathogenesis
  • About the PI
  • Personnel
  • Publications
  • Blog
  • Environment
  • Positions
  • Resources
  • Major Equipment
  • Donations
  • Comments
  • Contact Info
  • Internal Lab Resources