Our New Year's Eve manuscript acceptance, full text posting on ACS Infectious Diseases.  Congratulations to members of the Manetsch and Kirby Laboratories on on line publication of: "Development of CGS-15943 Adjunctives for the Disruption of Plasmid Maintenance in Multidrug Resistant E. coli." 

The manuscript describes structure activity relationship studies on a small molecule scaffold  CGS-15943.  The parent compound causes complete eviction of an IncFIA plasmid with highly selectivity in low, single-digit micromolar concentrations.   Kate Zulauf at al. previously described identification and characterization of several potent plasmid eviction compounds in our PNAS publication.  In our ACS Infectious Diseases manuscript, one portion of MInte's PhD thesis, CGS-15943 analogs were identified which were more potent than the parent compound, and with increased penetrance, An exciting step forward in our plasmid eviction work.

Forget CO2, Forget Temperature.
Selman Waksman  step over!  A big thumbs up! 

Ariane was immersed in antimicrobial resistance research on gram negatives in Ecuador prior to starting an internal medicine residency in 2022 at Salem Hospital and MGB.  In her spare time during her medical training, she will be continuing her investigation of gram-negative resistance mechanism in the Kirby lab.

BAARN would not be complete without antimicrobial medicinal chemistry.  Roman Manetsch and lab were there in force to highlight collaborative efforts on the streptothricin scaffold.

Some Press Releases:
Researchers Show an Antibiotic Discovered 80 Years Ago Is Effective Against Multi-Drug Resistant Bacteria
Neglected 80-year-old antibiotic is effective against multi-drug resistant bacteria
Vergeten antibioticum kan uitkomst zijn in strijd tegen multiresistente bacteriën

General areas include antimicrobial resistance, antimicrobial discovery, novel methods of treating antimicrobial resistant pathogens. 

A postdoctoral position is available immediately (4/2/2023) in the Laboratory of Ed Yu to study biology of the ribosome; mechanism of action of translational inhibitors; and interaction of small molecules with RNA in the Department of Pharmacology at Case Western Reserve. Projects are being performed collaboratively with the Kirby, Manetsch, and O'Doherty Laboratories. See recent manuscripts (PMC8805024, PMC8263017, PMC6974574)

The incoming research fellow will use x-ray crystallography/cryo-EM and other biophysical/biochemical techniques to elucidate the molecular mechanisms in these systems. Please find additional information about the Yu group and the outstanding training environment in the Case Western Department of Pharmacology website (https://case.edu/medicine/pharmacology/).

This position requires expertise in structural biology. The ideal candidate will have shown the ability to guide a project to completion, from protein expression to structural analysis. Knowledge of common structural programs (Phenix, Coot, Pymol) a must. Experience with cryo-electron microscopy sample preparation/operation/data analysis is highly desirable. Experience with single-molecule FRET and other biochemical and biophysical characterization methods would also be considered a strength.

Qualified applicants must have a PhD or MD/PhD degree; strong written, oral communication, and critical thinking skills are required. As a Postdoctoral Scholar, he/she will present data at departmental seminars, prepare manuscripts, and contribute preliminary data for grant proposals. Preference will be given to applicants with a recognized record of accomplishment as evidenced by scholarly publications in the field of bacteriology or structural biology. Send inquiries containing a cover letter, CV with list of publications and contact information to Dr. Edward Yu ([email protected]).

See also Ed' posting

Just published in mSphere!  Images from our manuscript show below.  Modeling of amotosalen efflux from the A. baumannii AdeB efflux pump.  Ref:
DOI: https://doi.org/10.1128/msphere.00673-22